The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates
Identifieur interne : 000024 ( Main/Exploration ); précédent : 000023; suivant : 000025The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates
Auteurs : Ao Sun [République populaire de Chine] ; Ke Xu [République populaire de Chine] ; Haifeng Liu [République populaire de Chine] ; Hua Li [République populaire de Chine] ; Yaohuang Shi [République populaire de Chine] ; Xiaoyan Zhu [République populaire de Chine] ; Tao Liang [République populaire de Chine] ; Xinyue Li [République populaire de Chine] ; Xianxia Cao [République populaire de Chine] ; Yanhong Ji ; Taijiao Jiang [République populaire de Chine] ; Chenqi Xu [République populaire de Chine] ; Xiaolong Liu [République populaire de Chine]Source :
- Scientific Reports [ 2045-2322 ] ; 2020.
Abstract
The recombination activating gene (RAG or RAG1/RAG2 complex)-mediated adaptive immune system is a hallmark of jawed vertebrates. It has been reported that RAG originated in invertebrates. However, whether RAG further evolved once it arose in jawed vertebrates remains largely unknown. Here, we found that zebrafish RAG (zRAG) had a lower activity than mouse RAG (mRAG). Intriguingly, the attenuated stability of zebrafish RAG2 (zRAG2), but not zebrafish RAG1, caused the reduced V(D)J recombination efficiency compared to mRAG at 37 °C which are the body temperature of most endotherms except birds. Importantly, the lower temperature 28 °C, which is the best temperature for zebrafish growth, made the recombination efficiency of zRAG similar to that of mRAG by improving the stability of zRAG2. Consistent with the prementioned observation, the V(D)J recombination of
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DOI: 10.1038/s41598-020-61019-w
PubMed: 32139788
PubMed Central: 7057966
Affiliations:
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Chine</country><wicri:regionArea>Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Li, Hua" sort="Li, Hua" uniqKey="Li H" first="Hua" last="Li">Hua Li</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1797 8419</institution-id><institution-id institution-id-type="GRID">grid.410726.6</institution-id><institution>State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences,</institution><institution>University of Chinese Academy of Sciences,</institution></institution-wrap>Shanghai, 200031 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Yaohuang" sort="Shi, Yaohuang" uniqKey="Shi Y" first="Yaohuang" last="Shi">Yaohuang Shi</name><affiliation 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Chenqi" uniqKey="Xu C" first="Chenqi" last="Xu">Chenqi Xu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1797 8419</institution-id><institution-id institution-id-type="GRID">grid.410726.6</institution-id><institution>State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences,</institution><institution>University of Chinese Academy of Sciences,</institution></institution-wrap>Shanghai, 200031 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Liu, Xiaolong" sort="Liu, Xiaolong" uniqKey="Liu X" first="Xiaolong" last="Liu">Xiaolong Liu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1797 8419</institution-id><institution-id institution-id-type="GRID">grid.410726.6</institution-id><institution>State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences,</institution><institution>University of Chinese Academy of Sciences,</institution></institution-wrap>Shanghai, 200031 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.440637.2</institution-id><institution>School of Life Science and Technology,</institution><institution>ShanghaiTech University,</institution></institution-wrap>Shanghai, 201210 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">The recombination activating gene (RAG or RAG1/RAG2 complex)-mediated adaptive immune system is a hallmark of jawed vertebrates. It has been reported that RAG originated in invertebrates. However, whether RAG further evolved once it arose in jawed vertebrates remains largely unknown. Here, we found that zebrafish RAG (zRAG) had a lower activity than mouse RAG (mRAG). Intriguingly, the attenuated stability of zebrafish RAG2 (zRAG2), but not zebrafish RAG1, caused the reduced V(D)J recombination efficiency compared to mRAG at 37 °C which are the body temperature of most endotherms except birds. Importantly, the lower temperature 28 °C, which is the best temperature for zebrafish growth, made the recombination efficiency of zRAG similar to that of mRAG by improving the stability of zRAG2. Consistent with the prementioned observation, the V(D)J recombination of <italic>Rag2</italic><sup>KI/KI</sup> mice, which zRAG2 was substituted for mRAG2, was also severely impaired. Unexpectedly, <italic>Rag2</italic><sup>KI/KI</sup> mice developed cachexia syndromes accompanied by premature death. Taken together, our findings illustrate that the evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cooper, Md" uniqKey="Cooper M">MD Cooper</name></author><author><name sortKey="Alder, Mn" uniqKey="Alder M">MN Alder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Flajnik, Mf" uniqKey="Flajnik M">MF Flajnik</name></author><author><name sortKey="Kasahara, M" uniqKey="Kasahara M">M Kasahara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schatz, Dg" uniqKey="Schatz D">DG Schatz</name></author><author><name sortKey="Swanson, Pc" uniqKey="Swanson P">PC Swanson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tonegawa, S" uniqKey="Tonegawa S">S Tonegawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hedrick, Sm" uniqKey="Hedrick S">SM Hedrick</name></author><author><name sortKey="Cohen, Di" uniqKey="Cohen D">DI Cohen</name></author><author><name sortKey="Nielsen, Ea" uniqKey="Nielsen E">EA Nielsen</name></author><author><name sortKey="Davis, Mm" uniqKey="Davis M">MM Davis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lewis, Sm" uniqKey="Lewis S">SM Lewis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lovely, Ga" uniqKey="Lovely G">GA Lovely</name></author><author><name sortKey="Sen, R" uniqKey="Sen R">R Sen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, Sf" uniqKey="Huang S">SF Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Yn" uniqKey="Zhang Y">YN Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carmona, Lm" uniqKey="Carmona L">LM Carmona</name></author><author><name sortKey="Fugmann, Sd" uniqKey="Fugmann S">SD Fugmann</name></author><author><name sortKey="Schatz, Dg" uniqKey="Schatz D">DG Schatz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Litman, Gw" uniqKey="Litman G">GW Litman</name></author><author><name sortKey="Cannon, Jp" uniqKey="Cannon J">JP Cannon</name></author><author><name sortKey="Dishaw, Lj" uniqKey="Dishaw L">LJ Dishaw</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huxley, J" uniqKey="Huxley J">J Huxley</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Boehm, T" uniqKey="Boehm T">T Boehm</name></author><author><name sortKey="Swann, Jb" uniqKey="Swann J">JB Swann</name></author></analytic></biblStruct><biblStruct><analytic><author><name 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Chakraborty</name></author><author><name sortKey="Sen, R" uniqKey="Sen R">R Sen</name></author><author><name sortKey="Desiderio, S" uniqKey="Desiderio S">S Desiderio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schatz, Dg" uniqKey="Schatz D">DG Schatz</name></author><author><name sortKey="Baltimore, D" uniqKey="Baltimore D">D Baltimore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ji, Yh" uniqKey="Ji Y">YH Ji</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Teng, G" uniqKey="Teng G">G Teng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caldwell, Rg" uniqKey="Caldwell R">RG Caldwell</name></author><author><name sortKey="Longnecker, R" uniqKey="Longnecker R">R Longnecker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fistonich, C" uniqKey="Fistonich C">C 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Liu</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Corneo, B" uniqKey="Corneo B">B Corneo</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Ji, Yanhong" sort="Ji, Yanhong" uniqKey="Ji Y" first="Yanhong" last="Ji">Yanhong Ji</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Sun, Ao" sort="Sun, Ao" uniqKey="Sun A" first="Ao" last="Sun">Ao Sun</name></noRegion><name sortKey="Cao, Xianxia" sort="Cao, Xianxia" uniqKey="Cao X" first="Xianxia" last="Cao">Xianxia Cao</name><name sortKey="Cao, Xianxia" sort="Cao, Xianxia" uniqKey="Cao X" first="Xianxia" last="Cao">Xianxia Cao</name><name sortKey="Jiang, Taijiao" sort="Jiang, Taijiao" uniqKey="Jiang T" first="Taijiao" last="Jiang">Taijiao Jiang</name><name sortKey="Li, Hua" sort="Li, Hua" uniqKey="Li H" first="Hua" last="Li">Hua Li</name><name sortKey="Li, Xinyue" sort="Li, Xinyue" uniqKey="Li X" first="Xinyue" last="Li">Xinyue Li</name><name sortKey="Liang, Tao" sort="Liang, Tao" uniqKey="Liang T" first="Tao" last="Liang">Tao Liang</name><name sortKey="Liu, Haifeng" sort="Liu, Haifeng" uniqKey="Liu H" first="Haifeng" last="Liu">Haifeng Liu</name><name sortKey="Liu, Xiaolong" sort="Liu, Xiaolong" uniqKey="Liu X" first="Xiaolong" last="Liu">Xiaolong Liu</name><name sortKey="Liu, Xiaolong" sort="Liu, Xiaolong" uniqKey="Liu X" first="Xiaolong" last="Liu">Xiaolong Liu</name><name sortKey="Shi, Yaohuang" sort="Shi, Yaohuang" uniqKey="Shi Y" first="Yaohuang" last="Shi">Yaohuang Shi</name><name sortKey="Shi, Yaohuang" sort="Shi, Yaohuang" uniqKey="Shi Y" first="Yaohuang" last="Shi">Yaohuang Shi</name><name sortKey="Xu, Chenqi" sort="Xu, Chenqi" uniqKey="Xu C" first="Chenqi" last="Xu">Chenqi Xu</name><name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name><name sortKey="Zhu, Xiaoyan" sort="Zhu, Xiaoyan" uniqKey="Zhu X" first="Xiaoyan" last="Zhu">Xiaoyan Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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